Researches have showed that Neotame is safe to child, pregnant women, lactating women, diabetics. No need special alert to phenylketonurics. Neotame has a rapid metabolism, can be excreted in the urine and stools, not accumulate in human body.

The major metabolic pathway is hydrolysis of the methyl ester byesterases that are present throughout the body, which yields de-esterified neotame and methanol. Because only trace amounts of neotame are needed to sweeten foods, the amount of methanol derived from neotame is much lower than that found in common food.